Partitioning and diffusion of proteins and linear polymers in polyacrylamide gels
نویسندگان
چکیده
منابع مشابه
Partitioning and diffusion of proteins and linear polymers in polyacrylamide gels.
The equilibrium partition coefficient (K) and diffusion coefficient (Dgel) of two proteins and two linear polymers were measured as a function of polymer content of a 2.7% cross-linked polyacrylamide (PA) gel. The gel concentration, expressed as a volume percentage of PA in the gel (phi), varied between 0 and 14%. The measurements were made by fluorescence spectroscopy; fluorescent dyes were co...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1996
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(96)79712-6